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Pathology

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pawsPathologyManual: Antibody Titer in Transfusion Service

Lonic Code

 

Test Name

Antibody Titer in Transfusion Service

Synonyms

Antibody titer, isohemagglutinin titer (immune anti-A or anti-B titer, prenatal titer, Rh titer, atypical antibody titer, AHG titer, IgG titer.

Test Consists

Antibody titration using appropriate RBC antigen.  Antibody detection and identification must be completed first.

Specimen Requirements

Blood

Volume and Container

Plain red stoppered tube or pink EDTA tube.

Storage and Transport

Send to Blood Bank.  Serum may be frozen in prenatal workups when a subsequent parallel titer is required.

Critical Values

In prenatal evaluations titer values above 32 or rising titer

Causes for Rejection

Improper labeling; wrong tube; severe hemolysis; insufficient quantity

Turn Around Time

24 hours

Availability

 

CPT / HCPCS Code

86886

Medicare Fee

 

Comments

 

Row Stamp

17,219,374

Last Updated

10/07/2003 10:59:27

Volume

10 mL

Minimum Volume

7 mL

Indications

To follow antibody levels in potential maternal-fetal blood incompatibility, to assess HDN, to assess IgG isoagglutinin levels in adults or infants, to assess antibody levels in transfusion reaction, or determine antibody concentration in a serum sample containing a known clinically significant antibody.

Professional Fee

 

Tech Charge

 

Total Fee

 

Special Instructions

Indicate reason for request i.e. HDN

Lab

Blood Bank  BI-1230  721-2731

Schedule

Testing Daily

Request Form

Complete a Blood Bank Miscellaneous Test Requisition Form. Indicate medical necessity.

Methodology

This is a semiquantitative method used to determine antibody concentration.  The reciprocal of the highest dilution of serum giving a 1+ reaction is referred to as the titer.

Patient Prep

None indicated.

Post Processing

 

Collection Guidelines

Label tube with patient name, MRN#, date collected, and phlebotomist initials

Reference Range

Not applicable

Limitations and Contraindications

Titers may not reflect the unborn infant''s condition; technical variables affect results'' completely reproducible results are not possible to achieve; can have biologic variability; results may differ by one dilution in tests performance; and titer values alone can be misleading.  Contraindictions: no antibody present, biologic father lacking corresponding antigen, antibody identified does not cause HDN, antibody identified not clinically significant.

Interpretation

Reported as the last dilution resulting in a 1+ reaction.

Additional Information

 

CPOE Test Name

 

Status

1

AUDIT_DEL

 

Attachments

Created at 9/8/2014 5:56 PM by Bradshaw, Keir
Last modified at 9/8/2014 5:56 PM by Bradshaw, Keir