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Pathology

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pawsPathologyManual: Antibody Identification

Lonic Code

 

Test Name

Antibody Identification

Synonyms

Antibody panel, panel testing, red cell antibody identification

Test Consists

Investigation of serum to identify antibody(ies) that were detected in the antibody screen procedure or crossmatch procedure.  Requires testing the serum against a panel of red cell specimens of known red cell antigen phenotypes for the major blood group systems.

Specimen Requirements

Blood

Volume and Container

Pink top (EDTA) tube. (Both a lavender and red stoppered tube may be requested if more tests are required for identification purposes).

Storage and Transport

Send to Blood Bank at room temperature

Critical Values

Significant findings include identification of clinically significant antibodies or antibody.

Causes for Rejection

Improper labeling; wrong tube; gross hemolysis; insufficient volume

Turn Around Time

24 hours

Availability

 

CPT / HCPCS Code

86870, 86885

Medicare Fee

 

Comments

 

Row Stamp

17,219,373

Last Updated

10/07/2003 10:59:27

Volume

10-20 mL

Minimum Volume

 

Indications

Necessary prior to transfusion to identify antibody or antibodies when the antibody screen test is positive.  May be done on eluates from red cells having a positive DAT test.  Necessary to work up obstetric patients for antenatal diagnosis of HDN.  To work up hemolytic anemia when the DAT and or indirect Coombs' test is positive.

Professional Fee

 

Tech Charge

 

Total Fee

 

Special Instructions

Indicate on requisition form diagnosis, transfusion history, recent drug history, and pregnancy history.

Lab

Blood Bank  BI-1230  721-2731

Schedule

Testing Daily

Request Form

 

Methodology

Immediate spin, 37C phase using LISS, then indirect Coombs' phase.  May require adsorption and elution procedures, selected panels, and other mediums for identification purposes.

Patient Prep

None

Post Processing

 

Collection Guidelines

Label tube(s) with patients name MRN#, date, and phlebotomists initials.  If blood components are required, label red stoppered tube using Fenwal Arm Band labeling system.  Refer to crossmatch test for labeling specifics.

Reference Range

Not applicable

Limitations and Contraindications

Antibody may be too weak to identify.  Panel cells may not contain specific antigen to antibody inserum.  Antibody may be to a low incidence antigen that may not be detected in the antibody screen test procedure.  May require sending specimen to a reference laboratory to identify and to obtain compatible units for transfusion.

Interpretation

 

Additional Information

Difficulty in crossmatching patients sensitized to red cell antigens may result in delay of blood availability.  Identified clinically significant antibodies known to cause HDN will be titered if identified in obstetric patients.

CPOE Test Name

Antibody Identification

Status

1

AUDIT_DEL

 

Attachments

Created at 9/8/2014 5:56 PM by Bradshaw, Keir
Last modified at 1/25/2016 3:19 PM by Payne, Valarie