Skip Ribbon Commands
Skip to main content

Pathology

:

pawsPathologyManual: Acid-Fast Smear for Mycobacteria

Lonic Code

 

Test Name

Acid-Fast Smear for Mycobacteria

Synonyms

AFB Smear, Stain Acid Fast for Mycobacteria

Test Consists

Mycobacterial [AFB] smear only; no culture performed.

Specimen Requirements

Sputum  early morning deep cough to produce lower respiratory specimen, bronchoalveolar lavage, bronchial brushing, sterile body fluids, tissue.

Volume and Container

Sterile sealed leak-proof container

Storage and Transport

Maintain at room temperature and transport to Microbiology Laboratory within one hour.

Critical Values

Phone report of smears positive for acid fast organisms.

Causes for Rejection

Use of nonsterile container; Delay in transport; Improper labeling; Insufficient volume

Turn Around Time

Result available within 24 hours, unless ordered as Emergent. Urgent test available upon request with same day results.

Availability

Non-urgent sent to ARUP reference laboratory; Urgent available same day

CPT / HCPCS Code

87206

Medicare Fee

 

Comments

 

Row Stamp

17,219,352

Last Updated

10/07/2003 10:59:27

Volume

> 1 mL

Minimum Volume

1 mL for most specimens; for CSF at least 5 mL; for urine at least 40 mL.

Indications

Suspected mycobacterial infection.

Professional Fee

 

Tech Charge

 

Total Fee

 

Special Instructions

 

Lab

Microbiology  BA-1672  721-2807

Schedule

24/7/365

Request Form

Microbiology & Infectious Serology requisition. Indicate medical necessity.

Methodology

Fluorochrome acid fast stain. If positive, a Kinyoun stain is performed to confirm.

Patient Prep

 

Post Processing

Observe for complications of collection procedure.

Collection Guidelines

Sputum -  See Collection Guidelines for Common Specimens;

Bronchalveolar lavage, bronchial brushings and transbronchial biopsy - Contact Pulmonary Medicine for collection;

Sterile body fluids - Collect using aseptic technique.

Reference Range

No acid fast organisms present.

Limitations and Contraindications

Weakly acid fast organisms may not be detected. Culture is more sensitive and is needed for organism identification and susceptibility testing.

Interpretation

 

Additional Information

If Nocardia is suspected, a weak acid fast stain is recommended.  Notify lab if a concentrated AFB smear is needed; these are done using a bleach cytocentrifuge technique .

CPOE Test Name

Smr Acid Fast

Status

1

AUDIT_DEL

 

Attachments

Created at 9/8/2014 5:56 PM by Bradshaw, Keir
Last modified at 1/28/2016 3:09 PM by Payne, Valarie